Traditional poultry farming, which supports over 75% of the workforce in Burkina Faso, is predominantly small-scale, run by women, and deeply integrated into local communities. It is a vital foundation for income generation and household nutrition and sustains livelihoods across generations. However, a range of challenges are encountered by poultry farmers, including limited access to health inputs and inadequate biosecurity measures, which increase the risk of community members to zoonotic diseases. Here we describe biosecurity measures in the village chicken flock in rural Burkina Faso.